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KMID : 0624620160490060319
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BMB Reports
2016 Volume.49 No. 6 p.319 ~ p.324
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A systematic study of nuclear interactome of C-terminal domain small phosphatase-like 2 using inducible expression system and shotgun proteomics
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Kang Na-Na
Koo Jae-Hyung
Wang Sen
Hur Sun-Jin
Bahk Young-Yil
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Abstract
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RNA polymerase II C-terminal domain phosphatases are newly emerging family of phosphatases that contain FCPH domain with Mg＋2-binding DXDX(T/V) signature motif. Its subfamily includes small CTD phosphatases (SCPs). Recently, we identified several interacting partners of human SCP1 with appearance of dephosphorylation and O-GlcNAcylation. In this study, using an established cell line with inducible CTDSPL2 protein (a member of the new phosphatase family), proteomic screening was conducted to identify binding partners of CTDSPL2 in nuclear extract through immunoprecipitation of CTDSPL2 with its associated. This approach led to the identification of several interacting partners of CTDSPL2. This will provide a better understanding on CTDSPL2.
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KEYWORD
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CTD phosphatase, CTDSPL2 inducible HEK293T cell line, CTDSPL2, Immunoprecipitation, Nuclear interactome
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